CENBIMO S.L

INTRODUCTION

Also known as Muramidase, Lysozyme catalyses the hydrolysis of some mucopolysaccharides in bacterial cell walls, specifically hydrolyzing between acetylmuramic acid and acetylglucosamine.

This enzyme is found in a variety of human cells but principally in tissular histiocytes, circulating granulocytes, monocytes and in glandular saliva and lacrimal cells.

The quantity of lysozyme produced by monocytes is greatly superior to that of polymorphonuclear neutrophil granulocytes.

Various point mutations in the lysozyme gene have been associated with non-neuropathic visceral amylodosis.

Histosonda Lysozyme consists of a fragment of single stranded DNA 358 nucleotides long that is targeted against lysozyme RNA,

The principle use of this probe is the typification and study of histiocytic cells (especially when they are activated and functioning) and the detection of monocytes in the bone marrow.

Due to the fact that in decalcified, paraffin embedded bone marrow biopsies it is not possible to clearly see the granulocytic cytoplasmic granuoles it is frequently very difficult to distinguish between myelocytes and monocytes. Histosonda Lysozyme permits a clean and clear differentiation of the bone marrow monocytic cells owing to the augmented transcription of the gene producing lysozyme. This permits the clear differentiation of chronic myelomonocytic leukemia from other types of chronic granulocytic leukemias.

INTENDED USE

For use in In Vitro Diagnosis.

Histosonda Lysozyme is useful for the typification and study of histiocytic cells (especially when they are activated and functioning) and the detection of monocytes in the bone marrow.

WARNINGS AND PRECAUTIONS

Histosonda Lysozyme has been designed for professional use in In Vitro Diagnosis and must be manipulated by qualified and accordingly trained personnel. In order to obtain the best results, the instructions contained in the manual must be followed. Any change to the indicated temperatures, times or any other step of the process can lead to poor results.

KIT COMPONENTS

The kit includes 20 single test tubes of lyophilized Histosonda Lysozyme.

Histosonda Lysozyme consists of a segment of single-stranded DNA complementary to expressed RNA with a length of 358 nucleotides.