CENBIMO S.L

INTRODUCTION

Cytomegalovirus belongs to the β-herpes virus family and globally affects the world population. Primary infection is often a flu-like process although there are known cases of greater severity that are clinically similar to mononucleosis.

Like other herpes viruses, after primary infection CMV persists in the host permanently as a latent infection.

Immunosuppression in patients can provoke reactivation of the virus giving rise to serious lesions in a multitude of organs such as the lungs, kidneys, central nervous system, gastrointestinal apparatus, endothelium and blood cells.

Patients with ulcerative colitis can be infected by CMV and treatment guidelines modified by this situation.

After infection, CMV first begins to transcribe the so-called ‘early’ genes. Of these genes, one known as β2.7 (2.7 kbases) is the most abundantly transcribed and accumulates during the entire infection until it makes up 20% of all mRNA transcribed by the virus. Because of this, the gene β2.7 is an ideal candidate for CMV identification.

CENBIMO has fabricated HISTOSONDA CMV which consists of a single strand of DNA with a sequence length of 288 nucleotides. This sequence is complementary to CMV gene β2.7 mRNA.

The technique of in situ hybridization presents numerous advantages over immunohistochemistry and unspecific results obtained when using antibodies can be avoided.

INTENDED USE

For use in In Vitro Diagnosis.

Histosonda Cytomegalovirus is intended for the detection of cells infected by CMV in any location found including: lymph node, central nervous system, retina, lung and intestine. This detection is carried out by in situ hybridization in paraffin-embedded, formalin-fixed histological sections.

WARNINGS AND PRECAUTIONS

Histosonda Cytomegalovirus has been designed for professional use in In Vitro Diagnosis and must be manipulated by qualified and accordingly trained personnel.

In order to obtain the best results, the instructions contained in the manual must be followed. Any change to the indicated temperatures, times or any other step of the process can lead to poor results.

KIT COMPONENTS

The kit includes 20 single test tubes of lyophilized .

Histosonda Cytomegalovirus. Histosonda Cytomegalovirus consists of a fragment of single stranded DNA of 288 nucleotides, complementary to CMV gene β2.7 mRNA. The probe DNA contains nucleotides that have been labeled with Digoxigenin.