CENBIMO S.L

CENBIMO S.L Manufactures Probes for in Situ Hybridization. (Single Stranded DNA Probes)

This new Technology patented by CENBIMO S.L allows CENBIMO to develop many different types of Probes. At the moment CENBIMO has developed 27 different types of Probes.

This New Technology brings many advantages beside previous technology, HISTOSONDA®:

Is Faster

The HistoSondas® have an incubation temperature of 62ºC. The elevated hermal energy generated allows the molecules to vibrate quicker therefore taking less time to find their target even though they are situated in a very viscose dextran containing media. The use of 62ºC as the incubation temperature for these probes therefore permits rapidity. HistoSonda® is the only commercial probe whose incubation period is just 30-60 minutes.

Is more specific

Due to their great lengths the HistoSondas® are more specific. It is statistically less probable to find shared sequence homology between long sequences.

Therefore the chances of the probe annealing with anything other than the target sequence are greatly reduced, increasing specificity. The HistoSonda® incubation temperature of 62ºC also permits that only chains with almost total sequence homology are annealed (an oligo will not anneal at this temperature).

Is more potent

The potency of the HistoSondas® is directly influenced by the number of labeled uridines in the DNA sequence. As the HistoSondas® have long sequences, the probability of large numbers of labelled uridines being incorporated is greatly increased, increasing the potency of the signal.

Is simple to use

The HistoSonda® hybridization procedure has been simplified to the maximum. It only uses one buffer during the entire technique (PBS). The use of formamide is not necessary and post-incubation washes with formamide have therefore been suppressed. The hybridization procedure can be carried out without needing to have specific knowledge of Molecular Biology.

Complicated apparatus is not necessary and revealing the probe can be performed automatically using any immunohistochemistry apparatus.

Is easy to transport and store:

The Histosondas® are supplied Lyophilized in single use tubes. Transport and storage can be carried out at room temperature and they have a long storage life. Freezers, refrigerators or any special type of care are not required.

The available List of actual probes is :

CEM -0001 HISTOSONDA® EBER
CEM- 0002 HISTOSONDA® HEMOGLOBIN ALPHA CHAIN
CEM -0003 HISTOSONDA® SERUM ALBUMIN
CEM -0004 HISTOSONDA® KAPPA LIGHT CHAIN
CEM- 0005 HISTOSONDA® LAMBDA LIGHT CHAIN
CEM- 0006 HISTOSONDA® ALPHA HEAVY CHAIN
CEM- 0007 HISTOSONDA® DELTA HEAVY CHAIN
CEM- 0008 HISTOSONDA® EPSILON HEAVY CHAIN
CEM- 0009 HISTOSONDA® GAMMA HEAVY CHAIN
CEM- 0010 HISTOSONDA® MU HEAVY CHAIN
CEM- 0011 HISTOSONDA® INSULIN
CEM- 0012 HISTOSONDA® GLUCAGON
CEM- 0013 HISTOSONDA® SOMATOSTATIN
CEM- 0014 HISTOSONDA® GASTRIN
CEM- 0015 HISTOSONDA® VASOACTIVE INTESTINAL PEPTIDE(VIP)
CEM- 0016 HISTOSONDA® THYROGLOBULIN
CEM- 0017 HISTOSONDA® CALCITONIN
CEM-0018 HISTOSONDA® CYTOMEGALOVIRUS (CMV)
CEM- 0019 HISTOSONDA® PANCREATIC PEPTIDE
CEM- 0020 HISTOSONDA® c-erbB2
CEM- 0021 HISTOSONDA® GHRL (Ghrelin\Obestatin)
CEM- 0022 HISTOSONDA® LISOZIMA
CEM-0023 HISTOSONDA® ALPHA-FETOPROTEINA
CEM-0024 HISTOSONDA® RNA RIBOSOMICO 18S HUMANO
CEM-0025 HISTOSONDA® GONADOTROFINA CORIONIC SUB. BETA
CEM-0026 HISTOSONDA® NEUROTENSIN
CEM-0027 HISTOSONDA® DUAL KAPPA-LAMBDA